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61.
AIM: To assess the efficacy of toltrazuril against the Eimeria spp. affecting brown kiwi (Apteryx mantelli).

METHODS: Droppings were collected from three brown kiwi, aged <6 months old, at a captive rearing facility in the North Island of New Zealand, between 22 February and 20 April 2017, on 14 sampling dates. Only droppings (n=30) that were excreted between 03:00 and 07:00, as determined using video surveillance, were included for analysis, reflecting the peak time for shedding of coccidial oocysts for brown kiwi. Oocysts were quantified in each sample and Eimeria species identified on the basis of oocyst morphology. All samples were collected between 2 and 10 days after the birds had been treated with 25?mg/kg toltrazuril.

RESULTS: Eimeria spp. oocysts were identified in 28/30 individual samples and on 14/14 sampling dates. Oocyst counts varied from 0 to 328,080 oocysts per gram (opg), and at least one oocyst count >10,000 opg was measured on 12/14 sampling dates. Three species of Eimeria were observed, with Eimeria apteryxii and E. kiwii most commonly encountered, whereas only one sample contained E. paraurii.

CONCLUSIONS AND CLINICAL RELEVANCE: In the three birds monitored at this research site, there was a high abundance of E. apteryxii and E. kiwii oocysts in droppings despite recent administration of toltrazuril. These results suggest that the populations of Eimeria spp. affecting brown kiwi at this location appear to possess an ability to survive exposure to toltrazuril. Toltrazuril is widely used at captive rearing facilities to limit the effects of coccidiosis in juvenile kiwi. If a lack of efficacy is confirmed, it will be necessary to investigate alternative treatment regimens alongside broader environmental management strategies.  相似文献   

62.
Objective To determine the effect of a long-acting local anaesthetic on the plasma cortisol response in calves dehorned using a scoop.
Design A physiological study with controls.
Procedure Seventy female calves, 3 to 4 months old, were divided into seven groups and dehorned by scoop with or without a long acting local anaesthetic (bupivacaine) which provides local anaesthesia for 3 to 4 h. In some treatment groups the local anaesthetic was given a second time, after 4 h, to give 8 h of anaesthesia. Blood samples were taken before and for 9 h after treatment and plasma cortisol concentrations were measured.
Results Scoop dehorning caused a marked rise in plasma cortisol concentration, which returned to pre-dehorning values after 7 h. The cortisol concentrations of calves given bupiva-caine were similar to those of control animals for 4 h after dehorning, but then a significant and protracted rise occurred, with concentrations returning to pretreatment values at about 9 h after dehorning. In calves given a second treatment of bupivacaine 4 h after dehorning, the plasma cortisol concentration remained similar to control values for 8 h after dehorning but then increased sharply.
Conclusions During its period of activity long-acting local anaesthetic prevented the rise usually seen after scoop dehorning in plasma cortisol concentrations; by inference it also alleviated the pain-induced distress caused by this procedure. However, when its effects had worn off the plasma cortisol values rose sharply indicating that animals still experienced pain-induced distress despite local anaesthetic being administered.  相似文献   
63.
The objective of this study was to determine the activity of steroid‐ and eicosanoid‐metabolizing enzymes in horses with varying BCSs. The BCSs of twenty non‐pregnant, anoestrous mares were determined prior to euthanasia, and tissue samples were collected from the liver, kidney, adrenal gland, ovary and endometrium. Cytochrome P450 1A (CYP1A), 2C (CYP2C), 3A (CYP3A) and uridine 5′‐diphospho‐glucuronosyltransferase (UGT) activities were determined using luminogenic substrates. The MIXED procedure of SAS was used to test the effect of BCS on enzyme activity and differences between tissues. Activity of CYP1A in adrenals was increased ( .05) in BCS 5 versus BCSs 4 and 6. Activity of CYP1A in the liver was increased (= .05) in BCS 4 versus BCSs 5 and 6. Activity of CYP1A was 100‐fold greater (< .0001) in the liver than in the adrenal, ovary and kidney. Activity of CYP2C was 100‐fold greater (< .0001) in the liver than in the adrenal, ovary and endometrium. Activity of CYP3A was only detectable in the liver. Activity of UGT in the kidney was decreased (= .02) in BCS 4 versus BCSs 5 and 6. Activity of UGT was threefold greater (< .0001) in the liver than in the kidney, whereas activity of UGT was ninefold greater (< .0001) in the kidney than in the ovary and endometrium. In general, BCS did not alter the activity of steroid‐ and eicosanoid‐metabolizing enzymes in horses. However, tissue differences in these enzymes indicated abundant hepatic metabolism in horses, which is similar to other livestock species.  相似文献   
64.
ABSTRACT

Aims: To characterise and classify wounds in sheep suspected to have been caused by attacks by kea (Nestor notabilis) (kea strike), and to report the prevalence of these wounds on five high country farms in the South Island of New Zealand.

Methods: Data were collected from farms between 28 August 2012 and 20 September 2013. Sheep were examined opportunistically immediately after shearing for signs of wounds caused by kea. The age and sex of sheep were also recorded. Wounds were measured and characterised as recent, healing, or healed, and the estimated true prevalence was calculated for each farm.

Results: Injuries consistent with kea strike wounds were identified in 70/13,978 (0.5%) sheep examined. The estimated true prevalence varied between farms, from 0 (95% CI?=?0–0.16) to 1.25 (95% CI?=?0.97–1.61)%. Of the 76 wounds identified, 61 (80%) were located in the lumbar region, and 74 (97%) consisted of full-thickness ulceration of the skin, one showed evidence of injury to muscle and one to bone. The median length of the 63 wounds measured was 6 (min 1, max?23.5) cm, and 10/63 (13%) were categorised as recently healed, 47/63 (62%) as healing, and 17/63 (22%) as recent wounds.

Conclusions: The results of this study show that kea strike on sheep was occurring at a low prevalence on the high country farms surveyed. The wounds identified were survivable, but the welfare impact of kea strike on sheep should be considered in balance with the conservation status of kea. There was clear variation in the prevalence of wounds attributed to kea strike between the farms but we were not able to identify the risk factors contributing to these differences. Future studies of kea strike should examine variables such as altitude, local kea density and distribution, and differences in kea strike management and husbandry practices, and should include high country farms without a history of kea strike.  相似文献   
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67.
Persistent Müllerian duct syndrome (PMDS) is a sex‐limited disorder in which males develop portions of the female reproductive tract. Important consequences of PMDS are cryptorchidism and its sequelae of infertility and increased risk of testicular cancer. Anti‐Müllerian hormone (AMH) and its receptor (AMHR2) induce the regression of the Müllerian ducts in male embryos. In Miniature Schnauzer dogs, the genetic basis has been identified as an autosomal recessive nonsense mutation in AMHR2, but the allele frequency of the mutation is unknown. Thus, the primary objective of this study was to estimate the prevalence of the AMHR2 mutation in North American Miniature Schnauzers, in order to ascertain the value of genetic testing in this breed. An additional objective was to determine whether mutations in AMH or AMHR2 were responsible for PMDS in a Belgian Malinois; this would aid development of a genetic test for the Belgian Malinois breed. Genomic DNA from 216 Miniature Schnauzers (including one known PMDS case) was genotyped for the AMHR2 mutation, and DNA from a single PMDS‐affected Belgian Malinois was sequenced for all coding exons of AMH and AMHR2. The Miniature Schnauzer cohort had an AMHR2 mutation allele frequency of 0.16 and a carrier genotypic frequency of 0.27. The genetic basis for PMDS in the Belgian Malinois was not determined, as no coding or splicing mutations were identified in either AMH or AMHR2. These findings support a benefit to AMHR2 mutation testing Miniature Schnauzers used for breeding or with cryptorchidism.  相似文献   
68.
The intensification of pastoral animal production results from several major developments including increased forage production and utilisation, diet supplementation, breeding animals to increase milk, meat or wool production, and changes in management. The impact of increased intensification on welfare will differ across species and systems. More intensive-grazing systems and the feeding of novel forages will underpin all moves to intensification. More intensive grazing generally reduces opportunities for shade and shelter. Improved nutrition will generally benefit welfare but competition for available feed may cause increased social pressure. Increased flock and herd size will be associated with a reduction in the human:animal ratio and less time to observe individual animals. Remote monitoring of activity and health might counter this impact.

Intensification of dairy production will result in larger herds, more year-round milking, robotic milking, use of housing and yards year round, and total mixed-ration feeding. Larger herds mean longer distances to walk to and from the dairy shed, and more lameness and less time to spend on self-maintenance activities such as grooming. Holding and feeding dairy cows on yards will cause an increase in lameness and mastitis and perhaps an increase in agonistic behaviour but will reduce time spent walking. Intensification of sheep production will involve increased flock size, increased fecundity, breeding from hoggets, and breeding ewes all year round. Housing during lambing might be considered appropriate, as would feeding to lift milk yields. Increased fecundity with an increase in triplets will increase lamb mortality rates, but housing ewes, when managed well, will result in reduced lamb mortality. Intensification of lamb finishing will be by improved nutrition. Intensification of beef production will include more breeding of heifers at 15 months, and more problems with dystocia.

Intensification of pastoral production will have positive and negative effects on animal welfare. The balance will be determined by the quality of management and stockmanship, and the pressure on businesses to be profitable.  相似文献   
69.
70.
AIMS: To quantify the range of attitudes and perceptions of cattle practitioners to painful procedures and conditions in cattle, to identify some of the demographic factors affecting those attitudes and perceptions, and to compare results from veterinarians based in New Zealand with those from the United Kingdom (UK) and Europe.

METHODS: A questionnaire, based on one used for similar surveys in Europe, was sent to all 455 members of the Society of Dairy Cattle Veterinarians (DCV) of the New Zealand Veterinary Association. This questionnaire included a section on demographic data, e.g. gender, year of graduation, and preveterinary school background, and a section which asked the respondents to rate on a scale of 1 to 10 how painful they thought 24 procedures and conditions would be without any analgesia.

RESULTS: A total of 166 questionnaires were returned, a response rate of 37%. From the range of procedures and conditions outlined in the questionnaire, those perceived as most painful were claw amputation, then Caesarean section, followed by surgery for left-displaced abomasum (LDA). The two least painful were mastitis with milk clots only, and the effect on a calf of dystocia.

There were significant differences between the pain scores assigned by men and women and by respondents who had graduated in different decades. Female respondents and more recent graduates tended to give a higher pain score for most conditions. These effects remained, even after the potential interaction between age and gender had been assessed. The effect on pain score of either background or time spent treating cattle was much less marked than the effect of age and gender.

Overall, the results were very similar to results from similar surveys undertaken in the UK and Europe.

CONCLUSIONS: The responses to this survey indicate that, as in the UK and Europe, dairy cattle practitioners in New Zealand have a wide range of attitudes to pain in cattle. The estimates of the severity of pain were subjective, but such a survey allows the majority opinion of a considerable number of informed assessors to be quantified.

CLINCAL RELEVANCE: The wide range of results suggests that more needs to be done to disseminate up-to-date knowl- edge of pain in cattle to veterinarians to ensure they provide appropriate levels of analgesia. In particular, the baseline data from this study can be used by practitioners to identify whether they are underestimating pain in cattle and, if they are, to assess whether they are using analgesia appropriately. Additionally, this dataset provides an informed and independent assessment of the pain associated with husbandry procedures, and could be used to better guide legislation concerning pain control of such procedures.  相似文献   
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